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Production, purification and properties of aPichia kluyverikiller toxin

โœ Scribed by E. J. Middelbeek; J. M. H. Hermans; C. Stumm

Book ID
104776387
Publisher
Springer Netherlands
Year
1979
Tongue
English
Weight
894 KB
Volume
45
Category
Article
ISSN
0003-6072

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โœฆ Synopsis

Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8-4.0 and 22-25 ยฐC. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly.

The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40ยฐC.

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โœ E. J. Middelbeek; H. H. A. M. Laar; J. M. H. Hermans; C. Stumm; G. D. Vogels ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 766 KB

The interaction between the killer toxin of Pichia kluyveri 1002 and cells of Saccharomyces eerevisiae SCF 1717 is strongly affected by the physiological state of sensitive cells. The killing effect is maximal for cells in the lag and early exponential phase of growth, whereas stationary cells are c