Production, purification and characterization of rat interferon
β Scribed by L. S. Lin; S. L. Abreu
- Publisher
- Springer Vienna
- Year
- 1979
- Tongue
- English
- Weight
- 547 KB
- Volume
- 62
- Category
- Article
- ISSN
- 1432-8798
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract We describe the production of marmoset lymphoblastoid cell interferon (IFN). Optimal yields of IFN were obtained when EBVβtransformed lymphoblastoid cell lines (LCL) at a cell density of 1 Γ 10^6^/ml were incubated with 100 HAU Sendai virus/ml for 24 h. Sendaiβvirusβinduced marmoset lym
Suwnary A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal columns. The IFN speci