Large scale production of human lymphoblastoid (Namalva) interferon II. Purification of interferon

✍ Scribed by Dino Marcus; Arye Lazar; Shaul Reuveny; Abraham Traub; Avshalom Mizrahi

• Publisher: Springer Netherlands
• Year: 1983
• Tongue: English
• Weight: 244 KB
• Volume: 5
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00132227

✦ Synopsis

Suwnary A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal columns. The IFN specific activity is increased rom 1 x 10 units/mg protein,

in the crude solution, to 2 x 107 and 2 x 10 is i units/mg protein, in the purified preparations obtained after polyclonal or monoclonal antibody columns, respectively.

The overall recovery of IFN in the procedure described is 50-55%.