Production of ψ-linolenic acid by the fungusMucor rouxiion cheap nitrogen and carbon sources

✍ Scribed by Anne-Marie Lindberg; Lena Hansson

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 281 KB
• Volume: 36
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00164693

✦ Synopsis

The production of 7-1inolenic acid (GLA) by the fungus Mucor rouxii CBS 416.77 was studied on low budget nitrogen and carbon sources, i.e. rape meal, cocos expeller and two types of yeast extract (nitrogen sources), and starch, starch hydrolysate, beet molasses and cocos expeller (carbon sources). As references, Difco yeast extract and glucose were used. In flask cultivations the three yeast extracts were fully interchangeable, while the Difco yeast extract (the most expensive of those tested) gave a higher productivity of G L A in fermentor cultures (14 rag. 1-1. h-1). The yield of lipids and G L A were increased in the order yeast extract < rape meal < cocos expeller. Thus the amount of lipid increased from 0.56 to 2.8 g. 1-1, and that of G L A from 0.15 to 0.33 g.1-1. Use of beet molasses or cocos expeller as carbon sources gave poor growth. Starch and starch hydrolysate resulted in better productivity of G L A than glucose (4.7 and 4.9 compared to 3.4 mg.1-1 • h -l ) .