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Production of tetraploid and homozygous diploid amphibians by suppression of first cleavage

✍ Scribed by Reinschmidt, Dana C. ;Simon, Stuart J. ;Volpe, E. Peter ;Tompkins, Robert

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 293 KB
Volume: 210
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402100115

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✦ Synopsis

Abstract

Tetraploid and genetically homozygous diploid Xenopus can be produced conveniently, in large numbers, by suppressing the first cleavage division of eggs through the application of hydrostatic pressure. The pressure‐induced disruption of the mitotic spindle during the first division of a diploid zygote of the African clawed toad results in a tetraploid embryo. A homozygous diploid embryo can be derived by inhibiting the first cleavage of a parthenogenetically activated haploid egg. Both tetraploid and homozygous diploid larvae are viable and successfully undergo metamorphosis.

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