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Production of androgenetic diploid axolotls by suppression of first cleavage

✍ Scribed by Gillespie, Laura L. ;Armstrong, John B.


Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
206 KB
Volume
213
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

Androgenetic diploid axolotls were produced by a combination of ultraviolet inactivation of the egg pronucleus and pressure suppression of the first cleavage division. Although survival was low, this technique will prove to be useful for producing completely homozygous animals in one step.


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✍ Reinschmidt, Dana C. ;Simon, Stuart J. ;Volpe, E. Peter ;Tompkins, Robert πŸ“‚ Article πŸ“… 1979 πŸ› John Wiley and Sons 🌐 English βš– 293 KB

## Abstract Tetraploid and genetically homozygous diploid __Xenopus__ can be produced conveniently, in large numbers, by suppressing the first cleavage division of eggs through the application of hydrostatic pressure. The pressure‐induced disruption of the mitotic spindle during the first division

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✍ Gillespie, Laura L. ;Armstrong, John B. πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 423 KB

## Abstract Androgenetic diploid axolotls were produced by ultraviolet inactivation of the egg pronucleus shortly after fertilization, followed by suppression of the first cleavage division by hydrostatic pressure or heat shock. After treatment at 14,000 psi for 8 minutes, diploidy was restored in