## Abstract Tetraploid and genetically homozygous diploid __Xenopus__ can be produced conveniently, in large numbers, by suppressing the first cleavage division of eggs through the application of hydrostatic pressure. The pressureβinduced disruption of the mitotic spindle during the first division
Production of androgenetic diploid axolotls by suppression of first cleavage
β Scribed by Gillespie, Laura L. ;Armstrong, John B.
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 206 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
Androgenetic diploid axolotls were produced by a combination of ultraviolet inactivation of the egg pronucleus and pressure suppression of the first cleavage division. Although survival was low, this technique will prove to be useful for producing completely homozygous animals in one step.
π SIMILAR VOLUMES
## Abstract Androgenetic diploid axolotls were produced by ultraviolet inactivation of the egg pronucleus shortly after fertilization, followed by suppression of the first cleavage division by hydrostatic pressure or heat shock. After treatment at 14,000 psi for 8 minutes, diploidy was restored in