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Production of supercoiled multimeric plasmid DNA for biopharmaceutical application

✍ Scribed by Carsten Voß; Torsten Schmidt; Martin Schleef; Karl Friehs; Erwin Flaschel


Book ID
118470946
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
275 KB
Volume
105
Category
Article
ISSN
0168-1656

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The topological structure of plasmid DNA can be characterized by capillary gel electrophoresis (CGE analysis)-an important tool for quality control and stability assessments in DNA storage or application. Hence, a large-scale manufacturing process was developed that allows the removal of undesired o

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## Abstract In this paper the influence of ammonium salt concentration on the production of pharmaceutical grade plasmid DNA from unfed high cell density batch culture on synthetic glycerol media is presented. Ammonium chloride in different concentrations (0 mmol dm^−3^, 18.7 mmol dm^−3^, 37 mmol d