Barley anther culture: Pretreatment on m
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Sarah L. Roberts-Oehlschlager; James M. Dunwell
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Article
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1990
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Springer
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English
โ 875 KB
A period of four days preincubation at 25 ยฐC on a medium containing mannitol was found to be superior to those pretreatments requiring incubation at 4 ยฐC. In addition, the yield of green plants was improved by orienting anthers 'flat' on the medium during mannitol preincubation, and reducing the num