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Production of pure and functional RNA for in vitro reconstitution experiments

✍ Scribed by Edelmann, Franziska Theresia; Niedner, Annika; Niessing, Dierk

Book ID
121227663
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
754 KB
Volume
65
Category
Article
ISSN
1046-2023

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RNA molecules are commonly produced in vitro by transcription, utilizing a DNA template, an RNA polymerase enzyme, and nucleoside triphosphate substrates (NTPs). In addition to the full-length RNA molecule coded for by the DNA template, significant amounts of shorter RNA molecules are produced. A si