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Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of Aeromonas hydrophila

✍ Scribed by Seung Hwan Lee; Dong Hyun Oh; Woo Suk Ahn; Young Lee; Jong-il Choi; Sang Yup Lee

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 80 KB
Volume: 67
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(20000120)67:2<240::aid-bit14>3.0.co;2-f

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✦ Synopsis

The newly screened Aeromonas hydrophila produces copolymer consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx). The characteristics of cell growth and polymer accumulation were examined using various carbon sources. P(3HB-co-3HHx) was produced from lauric acid and oleic acid only. P(3HB-co-3HHx) content can be increased by limitation of phosphorus. A maximal P(3HB-co-3HHx) content of 28.8 wt% could be obtained in flask culture. By applying the optimally designed nutrient feeding strategy, cell dry weight, P(3HB-co-3HHx) content, and 3HHx fraction obtained over the course of 43 h were 95.7 g/L, 45.2 wt%, and 17 mol%, respectively, resulting in a productivity of 1.01 g polyhydroxyalkanoate (PHA)/L и h.

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