Production of poly(3-hydroxybutyrate) by high cell density fed-batch culture of Alcaligenes eutrophus with phospate limitation

✍ Scribed by Hee Wook Ryu; Sei Kwang Hahn; Yong Keun Chang; Ho Nam Chang

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 76 KB
• Volume: 55
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19970705)55:1<28::aid-bit4>3.0.co;2-z

✦ Synopsis

High cell density fed-batch fermentation of Al-replacement applications (Brandl et al., 1990; Doi, caligenes eutrophus was carried out for the production 1990). Currently, ZENECA Bio Products produces coof poly(3-hydroxybutyrate) (PHB) in a 60-L fermentor.

polymers of PHB/PHV through a bacterial fermentation

During the fermentation, pH was controlled with NH 4 OH process under the trade name Biopol (Byrom, 1987; solution and PHB accumulation was induced by phos- , 1994; Holmes, 1985). Despite PHA's advantages phate limitation instead of nitrogen limitation. The glucose feeding was controlled by monitoring dissolved oxyover other polymer materials as mentioned above, its gen (DO) concentration and glucose concentration in high production cost has hindered its performance in the culture broth. The glucose concentration fluctuated the market (Lee, 1996). Improving the productivity of within the range of 0-20 g/L. We have investigated the the fermentation process and developing an efficient effect of initial phosphate concentration on the PHB prorecovery process will make it economically more feasiduction when the initial volume was fixed. Using an initial phosphate concentration of 5.5 g/L, the fed-batch fermenble (Hrabak, 1992; Lee, 1996).

Cox

tation resulted in a final cell concentration of 281 g/L, a PHA is usually formed and stored in the form of PHB concentration of 232 g/L, and a PHB productivity of intracellular inclusion bodies under unfavorable growth 3.14 g/L и h, which are the highest values ever reported conditions (Brandl et al., 1990; Doi, 1990). For example, to date. In this case, PHB content, cell yield from glucose, and PHB yield from glucose were 80, 0.46, and 0.38% oxygen deprivation, nitrogen deprivation, sulfate limita-(w/w), respectively.