✦ LIBER ✦

Production of mouse monoclonal antibodies using a continuous cell culture fermenter and protein G affinity chromatography

✍ Scribed by A. Zamboni; I. Giuntini; D. Gianesello; F. Maddalena; F. Rognoni; D. Herbst

Publisher: Springer
Year: 1994
Tongue: English
Weight: 873 KB
Volume: 16
Category: Article
ISSN: 0920-9069
DOI: 10.1007/bf00754610

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Increased production and recovery of sec

Increased production and recovery of secreted foreign proteins from plant cell cultures using an affinity chromatography bioreactor

✍ Eddie James; David R Mills; James M Lee 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 206 KB

Capture of human monoclonal antibodies f

Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography

✍ Ana M. Azevedo; A. G. Gomes; L. Borlido; I. F. S. Santos; D. M. F. Prazeres; M. 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 395 KB

## Abstract In this work, we investigated the feasibility of using phenyl boronate (PB) chromatography for the direct capture of monoclonal antibodies from a CHO cell supernatant. Preliminary results, using pure protein solutions have shown that PB media can bind to human antibodies, not only at st

Development and optimization of a single

Development and optimization of a single-step procedure using protein A affinity chromatography to isolate murine IgG1 monoclonal antibodies from hybridoma supernatants

✍ Gerhard Schuler; Manfred Reinacher 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 685 KB

Epitope mapping and the detection of tra

Epitope mapping and the detection of transmissible gastroenteritis viral proteins in cell culture using biotinylated monoclonal antibodies in a fixed-cell ELISA

✍ R. A. Simkins; L. J. Saif; P. A. Weilnau 📂 Article 📅 1989 🏛 Springer Vienna 🌐 English ⚖ 990 KB

Keratin 14 protein in cultured nonparenc

Keratin 14 protein in cultured nonparenchymal rat hepatic epithelial cells: Characterization of keratin 14 and keratin 19 as antigens for the commonly used mouse monoclonal antibody OV-6

✍ Hanne Cathrine Bisgaard; David C. Parmelee; Harold A. Dunsford; Salvatore Sechi; 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 694 KB

## Abstract We have recently reported that cell lines of nonparenchymal origin isolated from rat liver and pancreas, which have been suggested to be the progeny of a facultative stem cell compartment in vivo, express an unusual combination of keratins (K). These cell lines express K8 and K14 but no

Enhanced cell culture performance using

Enhanced cell culture performance using inducible anti-apoptotic genes E1B-19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary cells

✍ Bruno Figueroa Jr.; Eric Ailor; Douglas Osborne; J. Marie Hardwick; Mitchell Ref 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 495 KB 👁 3 views

## Abstract Mammalian cells are used for the production of numerous biologics including monoclonal antibodies. Unfortunately, mammalian cells can lose viability at later stages in the cell culture process. In this study, the effects of expressing the anti‐apoptosis genes, __E1B‐19K__ and __Aven__,