Increased production and recovery of secreted foreign proteins from plant cell cultures using an affinity chromatography bioreactor

Recovery of urokinase from integrated ma

Recovery of urokinase from integrated mammalian cell culture cryogel bioreactor and purification of the enzyme using p-aminobenzamidine affinity chromatography

✍ Vibha Bansal; Pradip K. Roychoudhury; Bo Mattiasson; Ashok Kumar 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 216 KB

## Abstract An integrated product recovery system was developed to separate urokinase from the cell culture broth of human kidney cells HT1080. Supermacroporous monolithic cryogels provided ideal matrices with respect to surface and flow properties for use as cell culture scaffold as well as for af