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Production of Milk-Clotting Protease fromBacillus subtilis

✍ Scribed by Kakoli Dutt; Pritesh Gupta; Saurabh Saran; Swati Misra; Rajendra Kumar Saxena

Book ID
107565193
Publisher
Springer-Verlag
Year
2009
Tongue
English
Weight
459 KB
Volume
158
Category
Article
ISSN
0273-2289

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## Abstract A milk‐clotting enzyme from __Bacillus subtilis__ K‐26 was purified by gel filtration and ion‐exchange chromatography resulting in a 24‐fold increase in specific activity with an 80% yield. Polyacrylamide gel electrophoresis and ultracentrifugel analysis revealed that the purified enzym