Production of L-methionine by immobilized pellets of Aspergillus oryzae in a packed bed reactor

✍ Scribed by Ying-Jin Yuan; Shu-hao Wang; Zheng-xiao Song; Rui-chang Gao

• Publisher: Wiley (John Wiley & Sons)
• Year: 2002
• Tongue: English
• Weight: 116 KB
• Volume: 77
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.615

✦ Synopsis

Abstract

Production of L‐methionine by immobilized pellets of Aspergillus oryzae in a packed bed reactor was investigated. Based on the determination of relative enzymatic activity in the immobilized pellets, the optimum pH and temperature for the resolution reaction were 8.0 and 60 °C, respectively. The effects of substrate concentration on the resolution reaction were also investigated and the kinetic constants (K~m~ and V~m~) of immobilized pellets were found to be 7.99 mmol dm^−3^ and 1.38 mmol dm^−3^ h^−1^, respectively. The maximum substrate concentration for the resolution reaction without inhibition was 0.2 mol dm^−3^. The L‐methionine conversion rate reached 94% and 78% when substrate concentrations were 0.2 and 0.4 mol dm^−3^, respectively, at a flow rate of 7.5 cm^3^ h^−1^ using the small‐scale packed bed reactor developed. The half‐life of the L‐aminoacylase in immobilized pellets was 70 days in continuous operation. All the results obtained in this paper exhibit a practical potential of using immobilized pellets of Aspergillus oryzae in the production of L‐methionine.

© 2002 Society of Chemical Industry