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Production of hydrogen peroxide by neutrophilic polymorphonuclear leukocytes in patients with diabetic nephropathy

✍ Scribed by Dr. Asako Watanabe; Yasuhiko Tomino; Ken-Ichi Yokoyama; Hikaru Koide

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
457 KB
Volume
7
Category
Article
ISSN
0887-8013

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✦ Synopsis

The production of hydrogen peroxide (H202) by neutrophilic polymorphonuclear leukocytes (PMN) after stimulation with phorbol myristate acetate (PMA), n-formyl-l-methionyl-lleucyl-l-phenylalanine (FMLP), aggregated human IgG, or Staphylococcus aureus was determined in 36 patients with non-insulin dependent diabetes mellitus (NIDDM). H202 production by PMN after stimulation was measured using flow cytometry. Thirty-six patients with NIDDM were divided into four stages as follows: 1) stage I: non-microalbuminuric stage; 2) stage II: microalbuminuric stage; 3) stage 111: proteinuric stage without impairment of renal function; and 4) stage IV: proteinuric stage with impairment of renal function. H202 production after PMA stimulation in all stages of NIDDM patients was higher than that in healthy controls. This increase of H202 production by PMN was particularly observed in stage IV of NIDDM patients after stimulation. Furthermore, H202 production in patients in stage IV was higher than that in patients with non-diabetic disease with impairment of renal function.

It appears that reactive oxygen species produced by PMN after stimulation under some conditions may play an important role in the progression of diabetic nephropathy. o 1993 Wiley-Liss, Inc.

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