Production of human parathyroid hormone by recombinant Escherichia coli TG1 on synthetic medium
β Scribed by Michael P.F. Harder; Ernst A. Sanders; Edgar Wingender; Wolf-Dieter Deckwer
- Book ID
- 119132128
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 571 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0168-1656
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Expression of human parathyroid hormone, hPTH(-1-84), by Escherichia coli N4830: pEX-PPTH was studied in controlled bioreactors. The hPTH is expressed, as a fusion protein under control of the bacteriophage )~PR promoter. In batch runs, low biomass concentrations but high specific hPTH productivitie
## Abstract Terrific broth, a complex medium containing a high content of yeast extract, was chosen to cultivate recombinant __Escherichia coli__ with the plasmid encoding the fusion protein gene of thioredoxin (Trx) and human parathyroid hormone (hPTH). The volumetric yield of TrxβhPTH fusion prot