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Production of human parathyroid hormone by recombinant Escherichia coli TG1 on synthetic medium

✍ Scribed by Michael P.F. Harder; Ernst A. Sanders; Edgar Wingender; Wolf-Dieter Deckwer


Book ID
119132128
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
571 KB
Volume
32
Category
Article
ISSN
0168-1656

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Expression of human parathyroid hormone, hPTH(-1-84), by Escherichia coli N4830: pEX-PPTH was studied in controlled bioreactors. The hPTH is expressed, as a fusion protein under control of the bacteriophage )~PR promoter. In batch runs, low biomass concentrations but high specific hPTH productivitie

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## Abstract Terrific broth, a complex medium containing a high content of yeast extract, was chosen to cultivate recombinant __Escherichia coli__ with the plasmid encoding the fusion protein gene of thioredoxin (Trx) and human parathyroid hormone (hPTH). The volumetric yield of Trx–hPTH fusion prot