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Large scale preparation of recombinant human parathyroid hormone 1–84 from Escherichia coli

✍ Scribed by Qinghai Liu; Jinping Lin; Meiyun Liu; Xinyi Tao; Dongzhi Wei; Xingyuan Ma; Shengli Yang


Book ID
116828604
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
326 KB
Volume
54
Category
Article
ISSN
1046-5928

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## Abstract Terrific broth, a complex medium containing a high content of yeast extract, was chosen to cultivate recombinant __Escherichia coli__ with the plasmid encoding the fusion protein gene of thioredoxin (Trx) and human parathyroid hormone (hPTH). The volumetric yield of Trx–hPTH fusion prot