Production of hepatitis B virus surface antigen containing pre-S1 and pre-S2 domains by Chinese hamster ovary cells
✍ Scribed by T. Lee; J. Inokoshi; M. Namiki; H Takeshima; S. Ōmura
- Publisher
- Springer Vienna
- Year
- 1989
- Tongue
- English
- Weight
- 828 KB
- Volume
- 106
- Category
- Article
- ISSN
- 1432-8798
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Sera from 11 patients with fulminant hepatitis B were tested for antibodies to translation products of the pre-S1 and pre-S2 regions of hepatitis B virus of IgM, IgA and IgG classes, as well as of IgA1, IgA2 and SIgA, with solid-phase enzyme immunoassays using native viral polypeptides. Antibodies t
Experimental design techniques were used to study the influence of the composition of the culture medium on the production of hepatitis B virus pre-S2 antigen by the methylotrophic yeast Hansenula polymorpha, pH, phosphoric acid, ammonium chloride and yeast extract concentrations were selected as ex
## Abstract The production and specific productivity of hepatitis B surface antigen (HBsAg) in recombinant Chinese hamster ovary (CHO) cells were increased by 81% and threefold, respectively, when supplemented with 1.5% dimethyl sulfoxide (DMSO) in the culture medium. To investigate the mechanism o