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Production of hepatitis b e antibody in epstein-barr virus-induced b lymphocyte cell lines

✍ Scribed by Dr. Yumiko Furuya; Masayasu Inoue; Yoshiya Yoshida; Namiko Yoshihara; Mitsuru Fakada

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
492 KB
Volume
9
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

B lymphocytes separated from anti‐HBe‐positive donors were established as lymphoblastoid cell lines by infection with EBV, but anti‐HBe in the culture supernatant from such lymphoblastoid cell lines could not be detected. The lymphoblastoid cell lines were rosetted with HBe antigen‐coupled SRBC to prepare cells for the production of specific anti‐HBe. Antibody activity in the culture supernatant against rosette forming cells was detected by RIA, ID, and R‐PHI tests during the first 1 to 4 wk, but not after 5 wk. The activity in the supernatant was not destroyed by treatment with 2‐mercaptoethanol, indicating that the antibody might be IgG.

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