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Epstein-Barr virus-induced IgE production in limiting dilution cultures of normal human B cells

✍ Scribed by Leonard D. Stein; Marcia A. Chan; Toshifumi Hibi; Hans-Michael Dosch

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 404 KB
Volume: 16
Category: Article
ISSN: 0014-2980
DOI: 10.1002/eji.1830160922

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✦ Synopsis

Epstein-Barr virus-induced IgE production in limiting dilution cultures of normal human B cells*

The induction of in vitro IgE production in human B cells from normal, nonatopic donors has been difficult and somewhat controversial. We report that IgE production is consistently observed in limiting dilution cultures of in vitro Epstein-Barr virus (EBV)-infected normal human B lymphocytes. The frequency of IgE-committed, EBV-responsive cells ranged from 1/810 to 1/10000 B lymphocytes, and it was similar in peripheral (blood, tonsil) and central (bone marrow) tissue sites. Poisson distribution analysis of these limiting dilution cultures suggested that IgE-committed B cells comprise 0.1-1% of all EBV-responsive B lymphocytes.

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## Abstract B lymphocytes separated from anti‐HBe‐positive donors were established as lymphoblastoid cell lines by infection with EBV, but anti‐HBe in the culture supernatant from such lymphoblastoid cell lines could not be detected. The lymphoblastoid cell lines were rosetted with HBe antigen‐coup