Production of haploids via anther culture inBrassica oleraceavar.Italica

✍ Scribed by W. A. Keller; K. C. Armstrong

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 549 KB
• Volume: 32
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00036875

✦ Synopsis

Culture

of broccoli (cv. Green Mountain) anthers at elevated temperatures (e.g. 35°C for two days) prior to maintenance at 25 "C stimulated microspore embryogenesis. Embryo yields were also increased by subjecting excised inflorescences to a short term, high temperature shock (i.e. 45°C for one hour followed by 40°C for three hours) prior to anther plating and by a ten-fold increase in the auxin level of the anther culture medium.

Plants were regenerated from microspore-derived embryos either directly through embryo culture or through the induction of organogenesis in explants of embryos which failed to develop on embryo culture medium. Approximately SO'% of the regenerates were haploids with the remainder considered to be spontaneously-doubled haploids.

Haploids were cloned and maintained as axial bud cultures for three years without the occurrence of ptoidy changes.

' Contribution

No. 698 Ottawa Research Station