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Production of doubled-haploid plants from tritordeum anther culture

✍ Scribed by P. Barceló; A. Cabrera; C. Hagel; H. Lörz

Publisher
Springer
Year
1994
Tongue
English
Weight
799 KB
Volume
87
Category
Article
ISSN
0040-5752

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✦ Synopsis

The effects of different media and cold pretreatment of spikes on the androgenic response and regeneration capacity from anther culture of tritordeum was studied. L5 medium gave the highest frequency of anther response. The frequency of cultures regenerating green or albino plantlets was not affected by the composition of the medium tested. Cold pretreatment of the spikes significantly increased the frequency of anther response and also the percentage of cultures giving albino plantlets. A mean of four green plants was obtained per 100 subcultured calli/embryos. The percentage of spontaneous chromosome doubling was only 1%. The addition of colchicine at 0.02% to the induction medium significantly increased the frequency of doubled haploids regenerated without any effect on regeneration capacity. This technique proved more efficient than a conventional chromosome-doubling method.

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