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Production of haploid-haploid chimeras by sperm injection in the sawfly, Athalia rosae (Hymenoptera)

โœ Scribed by Hatakeyama, Masatsugu ;Sawa, Masami ;Oishi, Kugao

Book ID
104656352
Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
389 KB
Volume
203
Category
Article
ISSN
1432-041X

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โœฆ Synopsis

Mature unfertilized eggs (oocytes) dissected from the ovary of the sawfly Athalia rosae (Hymenoptera) begin parthenogenetic development if exposed to distilled water and produce haploid males. Injection of sperm into mature oocytes through the anterior pole resulted in karyogamy in a fraction of cases which developed as diploid females. No haploid-haploid chimeras due to independent participation of the injected sperm in development were produced. When sperm were injected through the posterior pole, however, fertilization never occurred but haploid-haploid chimeras were produced in a smaller fraction of cases. Both egg nucleus-derived and injected sperm-derived nuclei contributed in forming the germ cells of the chimeric males.

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Participation of testicular spermatids in development upon intracytoplasmic injection into eggs of the sawfly,Athalia rosae (Insecta, Hymenoptera)
โœ Hatakeyama, Masatsugu; Nishimori, Yoshikatsu; Oishi, Kugao ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 321 KB ๐Ÿ‘ 3 views

Fertilization by intracytoplasmic injection of mature sperm into mature eggs has previously been achieved in the sawfly, Athalia rosae (Insecta, Hymenoptera). In the present study, we examined the potential of spermatids, premature male gametes, for participating in development. When round spermatid