Fertilization by micro-injection of cryopreserved sperm in the sawfly, Athalia rosae (Hymenoptera)
โ Scribed by Masatsugu Hatakeyama; Masami Sawa; Kugao Oishi
- Book ID
- 113182138
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 422 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0022-1910
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Mature unfertilized eggs (oocytes) dissected from the ovary of the sawfly Athalia rosae (Hymenoptera) begin parthenogenetic development if exposed to distilled water and produce haploid males. Injection of sperm into mature oocytes through the anterior pole resulted in karyogamy in a fraction of cas
Mature eggs dissected from the ovary of unmated females of Athalia rosae ruficornis Jakovlev (Hymenoptera, Tenthredinidae) can be activated to develop (into haploid parthenogenetic males) simply by exposing them to distilled water. These eggs, which are primary oocytes arrested at the first meiotic
Fertilization by intracytoplasmic injection of mature sperm into mature eggs has previously been achieved in the sawfly, Athalia rosae (Insecta, Hymenoptera). In the present study, we examined the potential of spermatids, premature male gametes, for participating in development. When round spermatid