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Production of Enzymatically and Immunologically Active Der f 1 in Escherichia coli

โœ Scribed by Takahashi, Kyoko; Takai, Toshiro; Yasuhara, Takaomi; Yuuki, Toshifumi; Ohtake, Yasuyuki; Yokota, Toyokazu; Okumura, Yasushi

Book ID
120675109
Publisher
S. Karger AG
Year
2000
Tongue
English
Weight
592 KB
Volume
122
Category
Article
ISSN
1018-2438

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Enzymatically active D 5 -3-ketosteroid isomerase (KSI) protein with a C-terminus his 6 -tag was produced following insoluble expression using Escherichia coli. A simple, integrated process was used to extract and purify the target protein. Chemical extraction was shown to be as effective as homogen