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Production and purification of active snowdrop lectin in Escherichia coli

✍ Scribed by Marian Longstaff; Kevin S. Powell; John A. Gatehouse; Romaan Raemaekers; Christine A. Newell; William D. O. Hamilton


Book ID
114428967
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
242 KB
Volume
252
Category
Article
ISSN
1432-1327

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A fusion protein was genetically engineered that contains an antimicrobial peptide, designated P2, at its carboxy terminus and bovine prochymosin at its amino terminus. Bovine prochymosin was chosen as the fusion partner because of its complete insolubility in Escherichia coli, a property utilized t