New soluble chromogenic substrates were prepared for specific and rapid assays of endo-l,4-/3-xylanases and endo-1 ,4+glucanases. A soluble beechwood 4-0-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R, and hydroxyethylcellulos was coupled to Ostazin brilliant red H-3B. The assays
Production of endo-1,4-β-glucanase and xylanase byTrichoderma reeseiimmobilized on polyurethane foam
✍ Scribed by Reetta Haapala; Susan Linko; Elke Parkkinen; Pirkko Suominen
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 431 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0951-208X
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✦ Synopsis
Endo-I,4-fI-glucanase and xylanase were produced by Trichodenna reesei immobilized on polyurethane foam using lactose as the main carbon source. The most porous carrier was found to be the best of those tested. The nitrogen source and KHzPO, concentration of the production medium had a marked effect on culture pH during the course of fermentation and, consequent&, on xylanase activity. An increase in lactose concentration from 7 to 27 gll resulted in an increase in endoglucanase activity (max. 730 VJml). xylanase activity (max. 3350 U/ml) and filter paper activity (max. 3.0 FPUIml).
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The production of endo-beta-1,4-glucanase by a Bacillus strain isolated from a hot spring in Zimbabwe was studied in batch culture, chemostat culture, and carbon dioxide-regulated auxostat (CO2-auxostat). The bacteria produced the enzyme in the presence of excess glucose or sucrose, but not under ca