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Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate by fed-batch culture ofAlcaligenesSP. SH-69

โœ Scribed by Y. H. Rhee; J-H Jang; P. L. Rogers

Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
473 KB
Volume
15
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate [poly(3HB-co-3HV)] by fed-batch culture ofAlcaligenes sp. SH-69 was investigated using glucose as a sole carbon source. Synthesis of poly(3HB-co-3HV) during the polymer accumulation stage was favored under dissolved oxygen tension at 20% and C/N ratio (mol glucose/mol ammonium) of 23.1. When conditions were optimal, 36 g liter -I of poly(3HBco-3HV) containing 3.0 mol% of 3HV was produced. Decreasing C/N ratio resulted in an increase of 3HV fraction in the copolymer to a maximum level of 6.3 mol%.

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High cell density fed-batch fermentation of Al-replacement applications (Brandl et al., 1990; Doi, caligenes eutrophus was carried out for the production 1990). Currently, ZENECA Bio Products produces coof poly(3-hydroxybutyrate) (PHB) in a 60-L fermentor. ## polymers of PHB/PHV through a bacterial