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Production of colony-stimulating factors by Hodgkin cell lines

✍ Scribed by Heinz Burrichter; Wolfgang Heit; Michael Schaadt; Hartmut Kirchner; Volker Diehl

Publisher
John Wiley and Sons
Year
1983
Tongue
French
Weight
505 KB
Volume
31
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

This work was performed with L 428, a cell line established in 1978 from a patient with Hodgkin's disease. These in vitro cells represent counterparts of in vivo Hodgkin and Sternberg‐Reed cells. L 428 and its derived sublines produce a significant amount of colony‐stimulatling factor (CSF) compared to standard preparations of CSF (fetal liver conditioned medium). Hodgkin‐cell‐derived conditioned medium, tested in cord‐blood assays and in semi‐solid agar systems, induced myeloproliferation predominantly. Production of CSF is independent of fetal calf serum concentration in the cultures. L 428‐conditioined medium could provide an excellent source for characterization and purification of granulocyte CSF.

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