Production of cellulase and β-glucosidase activity during growth of the actinomyceteMicromonospora chalcaeon cellulose-containing media
✍ Scribed by J. Gallagher; A. Winters; N. Barron; L. McHale; A. P. McHale
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 294 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
The actinomycete, Micromonospora chalcae produced an extracellular cellulase system when grown on cellulose-containing media. Cellulase activity reached a maximum after 8 days and the P-glucosidase activity was maximal within 16 to 18 days. P-Glucosidase activity was isolated and had a Km of 0.25mM for the substrate p-nitrophenyl-P-D-glucoside. The activity was optimal at pH 7.25 and 52°C.
📜 SIMILAR VOLUMES
We report-the partial characterization of a 13-glucosidase produced during growth of the thermotolerant yeast, K. marxianus IMB3 on lactose-containing media at 45°C. The enzyme had Km values of 1.1mM and 14.SmM fbr the substrates p-nitrophenyl-13-D-glucoside and ceilobiose, respectively. The enzyme