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Production of cellulase and β-glucosidase activity during growth of the actinomyceteMicromonospora chalcaeon cellulose-containing media

✍ Scribed by J. Gallagher; A. Winters; N. Barron; L. McHale; A. P. McHale


Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
294 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis


The actinomycete, Micromonospora chalcae produced an extracellular cellulase system when grown on cellulose-containing media. Cellulase activity reached a maximum after 8 days and the P-glucosidase activity was maximal within 16 to 18 days. P-Glucosidase activity was isolated and had a Km of 0.25mM for the substrate p-nitrophenyl-P-D-glucoside. The activity was optimal at pH 7.25 and 52°C.


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Partial characterization of β-glucosidas
✍ N. Barron; R. Marchant; L. McHale; A. P. McHale 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 280 KB

We report-the partial characterization of a 13-glucosidase produced during growth of the thermotolerant yeast, K. marxianus IMB3 on lactose-containing media at 45°C. The enzyme had Km values of 1.1mM and 14.SmM fbr the substrates p-nitrophenyl-13-D-glucoside and ceilobiose, respectively. The enzyme