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Production of bicyclic and tricyclic triterpenes by mutated squalene-hopene cyclase

✍ Scribed by Catherine Pale-Grosdemange; Thorsten Merkofer; Michel Rohmer; Karl Poralla

Book ID
104262031
Publisher
Elsevier Science
Year
1999
Tongue
French
Weight
207 KB
Volume
40
Category
Article
ISSN
0040-4039

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✦ Synopsis

Mutagenesis of Tyr420 to Ala in the catalytic cavity of squalene-hopene cyclase of Alicyclobacillus acidocaldarius resulted in an altered product spectrum. Besides hopene and diplopteml, this mutant prudueed significant amounts of the bicyclic ct-and y-polypodatetraenes and minor amounts of the tricyclic 13a0-I)-malabaricatriene.

πŸ“œ SIMILAR VOLUMES

Altered product pattern of a squalene-ho
Altered product pattern of a squalene-hopene cyclase by mutagenesis of active site residues
✍ Thorsten Merkofer; Catherine Pale-Grosdemange; Karl Ulrich Wendt; Michel Rohmer; πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 French βš– 177 KB

Amino acid residues lining the catalytic cavity of squalene-hopene cyclase of Alicyclobacillus acidocaldarius have been mutated. Alterations of His451 to Ala and Trp489 to Ala resulted in reduced enzymatic activity, while the product patterns were identical to that of the wild-type. Mutation of Phe6