Bicyclic triterpenes as new main products of squalene–hopene cyclase by mutation at conserved tyrosine residues

Production of bicyclic and tricyclic tri

Production of bicyclic and tricyclic triterpenes by mutated squalene-hopene cyclase

✍ Catherine Pale-Grosdemange; Thorsten Merkofer; Michel Rohmer; Karl Poralla 📂 Article 📅 1999 🏛 Elsevier Science 🌐 French ⚖ 207 KB

Mutagenesis of Tyr420 to Ala in the catalytic cavity of squalene-hopene cyclase of Alicyclobacillus acidocaldarius resulted in an altered product spectrum. Besides hopene and diplopteml, this mutant prudueed significant amounts of the bicyclic ct-and y-polypodatetraenes and minor amounts of the tric