Production of aromatic d-amino acids from α-keto acids and ammonia by coupling of four enzyme reactions
✍ Scribed by Hee-Sung Bae; Seung-Goo Lee; Seung-Pyo Hong; Mi-Sun Kwak; Nobuyoshi Esaki; Kenji Soda; Moon-Hee Sung
- Book ID
- 114396372
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 232 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1381-1177
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The inhibition of D-Amino Acid Oxidase by a-Keto Acid, product of the reaction with D-Amino Acid is described for the first time. Inhibition of the enzyme by o-Ketoy-Methiolbutyric Acid (4-methylthio-2-oxobutanoic acid), product of the reaction with D-Methionine, was studied. From the results obtain
This study reports on the development of a bioreactor for the production of a-keto acids from D,L-or D-amino acids using Rhodotorula gracilis o-amino acid oxidase. D-Amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank r