𝔖 Bobbio Scriptorium
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Production of antibodies to tetrahydrocannabinol as the basis for its radioimmunoassay

✍ Scribed by TEALE, J. D.; FORMAN, ELVYNE J.; KING, L. J.; MARKS, V.

Book ID
109690327
Publisher
Nature Publishing Group
Year
1974
Tongue
English
Weight
232 KB
Volume
249
Category
Article
ISSN
0028-0836

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Rubratoxin B was coupled to ovalbumin using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide HC1 (ECDI) in high concentration at pH 8.0. Under these conditions it was possible to couple 13 moles of rubratoxin B per mole of ovalbumin. The conjugate was used for immunization of rabbits, and anti-rubratox