✦ LIBER ✦

Production of a thermostable ATPase by the facultative thermophile,Bacillus coagulans

✍ Scribed by C. Edwards; M. V. Jones

Publisher: Springer
Year: 1983
Tongue: English
Weight: 267 KB
Volume: 135
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00419486

No coin nor oath required. For personal study only.

✦ Synopsis

The properties of the ATPase in the facultative thermophile, Bacillus coagulans, grown at thermophilic or mesophilic temperatures were similar. Arrhenius plots did not show discontinuities indicative of thermoadaptation. Magnesium stimulation of the enzyme was dependant on the assay temperature but independant of the growth temperature. The ATPase in cells grown at 35~ or 55~ was equally thermostable at 65~

In contrast, the ATPase from the mesophile, Bacillus megaterium (Tma x = 42 ~ C) was completely inactivated at 55~ in 5min.

📜 SIMILAR VOLUMES

The respiratory chain of the facultative

The respiratory chain of the facultative thermophile,Bacillus coagulans

✍ A. S. Ball; C. Edwards 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 683 KB

Production of extracellular thermostable

Production of extracellular thermostable pullulanase by an amylolytic obligately thermophilic soil bacterium,Bacillus stearothermophilusKP 1064

✍ Yuzuru Suzuki; Masaharu Chishiro 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 560 KB

Production of thermostable xylanase by a

Production of thermostable xylanase by a thermophilic fungus, Thermoascus aurantiacus

✍ Ernest K.C. Yu; Larry U.L. Tan; Maria K.-H. Chan; Lise Deschatelets; John N. Sad 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 875 KB

Production of a thermostable uricase by

Production of a thermostable uricase by a novel Bacillus thermocatenulatus strain

✍ Walid A. Lotfy 📂 Article 📅 2008 🏛 Elsevier Science 🌐 English ⚖ 160 KB

Thermostability and electron transfer ac

Thermostability and electron transfer activity of the ferredoxin from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii

✍ Shigetoshi Aono; Norio Fukuda; Ichiro Okura 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 623 KB

The Azorobucrer-type 7Fe ferredoxin from Bacillus schlegelii was active as an electron carrier in the reduction system of cyt. c consisting of NADPH, FNR and cytochrome c (cyt. c). The only [ 3Fe\_4S] cluster in the Fd molecule acts as the active site for the reduction of cyt. c. The activity of the

A Novel Fluorescence Competitive Assay f

A Novel Fluorescence Competitive Assay for Glucose Determinations by Using a Thermostable Glucokinase from the Thermophilic Microorganism Bacillus stearothermophilus

✍ Sabato D'Auria; Nicolas DiCesare; Maria Staiano; Zygmunt Gryczynski; Mose' Rossi 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 99 KB

We describe the use of a thermostable glucokinase in a novel competitive fluorescence assay for glucose. Glucokinase from Bacillus stearothermophilus (BSGK) was found to retain enzymatic activity in solution for over 20 days. The single cysteine residue in BSGK, which is near the active site, was la