Previously steam explosion had been used to enhance the enzymatic hydrolysis of lignocellulosic substrates to glucose. The conditions for pretreating aspen wood chips were optimized so that highest amounts of undegraded hemicellulose could be obtained after washing the steam exploded chips. The hemi
Production of 2,3-butanediol from HF-hydrolyzed aspen wood
โ Scribed by E. K. C. Yu; L. Deschatelets; N. Levitin; J. N. Saddler
- Publisher
- Springer Netherlands
- Year
- 1984
- Tongue
- English
- Weight
- 267 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0141-5492
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โฆ Synopsis
Hydrolyzates from hydrogen fluoride (HF) treated aspenwood were predominantly composed of oligosaccharides which are not readily utilized by Klebsiella pneumoniae. Attempts at further hydrolyzing these ollgosaccharides using a variety of glycolytic and xylanolytic enzymes (i.e., amylases, cellulases, and xylanases) were only partially successful. When a post-hydrolysis step was carried out using 3% H2SO 4, significant amounts of the component monosaccharides were detected. Sugars released by acid or enzymatic hydrolysis of the HF treated aspenwood were utilized by K. pneumoniae for the production of butanediol and ethanol.
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Of four organisms tested in semi-synthetic medium for the production of 2,3-butanediol from lactose, Klebsiella bneumoniae These results indicate that lactose utilization may be a limiting step in the fermentation process.