Production and regeneration of protoplasts from the mycorrhizal fungusSuillus granulatus

✍ Scribed by E. S. Dias; E. F. Araújo; W. V. Guimarães; R. M. C. Muchovej

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 360 KB
• Volume: 12
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00327726

✦ Synopsis

Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3×10(7)/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was MgSO4 (0.7 M). To optimize protoplast release and regeneration an enzyme (Novozym 234) concentration 1.7 mg/ml was chosen, with a digestion time of 1 to 2 h. Regenerated colonies formed mycorrhizae within 60 days after inoculation in Pinus caribaea var. hondurensis seedlings.