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Production and purification of lentiviral vectors

✍ Scribed by Tiscornia, Gustavo; Singer, Oded; Verma, Inder M


Book ID
109945967
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
136 KB
Volume
1
Category
Article
ISSN
1750-2799

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## Abstract The use of lentiviral vectors as gene delivery vehicles has become increasingly popular in recent years. The growing interest in these vectors has created a strong demand for large volumes of vector stocks, which entails the need for scaleable vector manufacturing procedures. In this wo

Optimized production and concentration o
✍ Nadya al Yacoub; Malgorzata Romanowska; Natalie Haritonova; John Foerster πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 331 KB

## Abstract Generation of high titer lentiviral stocks and efficient virus concentration are central to maximize the utility of lentiviral technology. Here we evaluate published protocols for lentivirus production on a range of transfer vectors differing in size (7.5–13.2 kb). We present a modified