Production and preliminary characterization of RNA-depolymerase fromTrichoderma harzianum
β Scribed by E. S. Vasileva-Tonkova
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 229 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1573-0972
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β¦ Synopsis
Trichoderma harzianum produced RNA-depolymerase with maximum activity after 72 to 120 h of growth. Addition of K2HPO4 repressed enzyme production by the fungus. The optimal activity was at pH 7.8 and 40 to 50Β°C. The enzyme was stable at pH 3.2 to 9.0 and 80% of activity remained after 60 min at 40Β°C. EDTA and p-chloromercuribenzoate had no effect on the enzyme activity.
π SIMILAR VOLUMES
The microheterogeneity of trichorzianine-A (TA) from Trichoderma harzianum was studied using single-stage mass spectrometry and high-energy collision-induced dissociation (CID) tandem mass spectrometry. Based on the electrospray ionization and the liquid secondary ion single-state mass spectra, the