Microheterogeneity characterization of a trichorzianine-A mixture fromTrichoderma harzianum

✍ Scribed by Pócsfalvi, Gabriella; Scala, Felice; Lorito, Matteo; Ritieni, Alberto; Randazzo, Giacomino; Ferranti, Pasquale; Vékey, Károly; Malorni, Antonio

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 380 KB
• Volume: 33
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199802)33:2<154::aid-jms620>3.0.co;2-0

✦ Synopsis

The microheterogeneity of trichorzianine-A (TA) from Trichoderma harzianum was studied using single-stage mass spectrometry and high-energy collision-induced dissociation (CID) tandem mass spectrometry. Based on the electrospray ionization and the liquid secondary ion single-state mass spectra, the main components with eight di †erent molecular masses were identiÐed in the mixture. Five contain phenylalaninol and three a tryptophanol C-terminal amino alcohol residue. The tandem mass spectra taken on the protonated molecular ion of these components (MH') show that most of them contain one main and one or more minor sequence analogues. For the microheterogeneity characterization of this mixture, a previously described method was used based on high-energy CID studies of the di †erent MH', ions and their satellite ions with »14 and »28 mass number di †erences. In y 7 , b 12 addition to the nine known sequence variants, Ðve new trichorzianines were sequenced from the mixture. It was found, furthermore, that amino acid exchange can occur not only at positions 5, 14, 16 and 19 but also at positions 2, 3, 4, 7, 8 and 9. 1998