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Production and partial characterisation of extracellular lipase fromAspergillus niger

✍ Scribed by Dimitris G. Hatzinikolaou; John B. Macris; Paul Christakopoulos; Dimitris Kekos; Fragiskos N. Kolisis; George Fountoukidis

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
465 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

The production and certain kinetic characteristics of extracellular lipase from Asperbai/!us niger were investigated. It was possible to substantially enhance the activity of excreted lipase by optimising the interaction between carbon and nitrogen sources applying a two-parameter complete experimental design and response surface analysis. The enzyme was partially purified and a number of kinetic characteristics such as optimum pH and temperature, thermal and pH stability and K, were determined and discussed.'The elevated levels of lipase activity (40.5 U/ml) found in this work competed favourably with most of those reported for lipase hyperproducing fungi.

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