Production and characterization of extracellular lipase fromCalvatia gigantea

✍ Scribed by Paul Christakopoulos; Constantina Tzia; Dimitris Kekos; Basil J. Macris

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 415 KB
• Volume: 38
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00174467

✦ Synopsis

A number of factors affecting production of extracellular lipase by the edible fungus Calvatia gigan- tea were investigated. Consecutive optimization of carbon and nitrogen sources, initial pH of culture medium and growth temperature resulted in an increase in lipase activity of 87%. Under optimum conditions, activities as high as 22.4 units ml-1 of culture medium were obtained, competing favourably with most activities reported for other lipase hyperproducing microorganisms. The enzyme was optimally active at pH 7.0 and 30°C and had, at optimum pH, half-lives of 75.7 and 22.9 rain at 45 and 55 ° C. Both high activity and kinetic characteristics of the enzyme make this process worthy of further investigation.