Production and Glycosylation of Recombinant β-Interferon in Suspension and Cytopore Microcarrier Cultures of CHO Cells
✍ Scribed by Maureen Spearman; Jose Rodriguez; Norm Huzel; Michael Butler
- Book ID
- 109387126
- Publisher
- American Institute of Chemical Engineers
- Year
- 2008
- Tongue
- English
- Weight
- 308 KB
- Volume
- 21
- Category
- Article
- ISSN
- 8756-7938
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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37°C until viable cell concentration reached 1 × 10^7^ c
## Abstract Both the macroheterogeneity of recombinant human IFN‐γ produced by CHO cells and intracellular levels of nucleotides and sugar nucleotides, have been characterized during batch and fed‐batch cultures carried out in different media. Whereas PF‐BDM medium was capable to maintain a high pe