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Effect of amplification ofdhfrandlacZ genes on growth and β-galactosidase expression in suspension cultures of recombinant CHO cells

✍ Scribed by Man Bock Gu; Jeffrey A. Kern; Paul Todd; Dhinakar S. Kompala

Book ID
110546916
Publisher
Springer
Year
1992
Tongue
English
Weight
688 KB
Volume
9
Category
Article
ISSN
0920-9069

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37°C until viable cell concentration reached 1 × 10^7^ c