Production and characterization of antibodies directed against organophosphorus nerve agent VX
β Scribed by Jean-Marc Grognet; Thierry Ardouin; Michel Istin; Alain Vandais; Jean-Pierre Noel; Ghassoub Rima; Jacques Satge; Christian Pradel; Henri Sentenac-Roumanou; Claude Lion
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 618 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0340-5761
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Haptens bearing phosphorane and c~-hydroxyphosphinate structures have been designed and synthesized for the selection of monoclonal antibodies with phosphatase like activity in order to detoxify highly toxic organophosphorus chemical weapons such as VX.
## Abstract Hybridoma CE7 produces a murine antibody (yl/k) which binds to a 190βkDa cellβsurface glycoprotein of human neuroblasβtoma. Because of its tumor specificity, it has been used routinely in clinical pathology to confirm diagnosis of neuroblastoma. We have Isolated the gene segments coding
MAb were derived from mice immunized with cells of the human neuroblastoma line IMR-32. Five hybridomas were selected according to their selective binding to human cell lines, tumors and normal tissues. One of them, CE7, reacted with all sympatho-adrenomedullary cells (neuroblastoma, ganglioneurobla