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Production and characterization of a genetically engineered anti-caffeine camelid antibody and its use in immunoaffinity chromatography

✍ Scribed by Elliott J. Franco; Gregory J. Sonneson; Thomas J. DeLegge; Heike Hofstetter; James R. Horn; Oliver Hofstetter


Book ID
108161033
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
781 KB
Volume
878
Category
Article
ISSN
1873-376X

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Rubratoxin B was coupled to ovalbumin using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide HC1 (ECDI) in high concentration at pH 8.0. Under these conditions it was possible to couple 13 moles of rubratoxin B per mole of ovalbumin. The conjugate was used for immunization of rabbits, and anti-rubratox