Production of anti-rubratoxin antibody a
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Robert M. Davis; Stanley S. Stone
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Article
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1979
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Springer Netherlands
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English
β 372 KB
Rubratoxin B was coupled to ovalbumin using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide HC1 (ECDI) in high concentration at pH 8.0. Under these conditions it was possible to couple 13 moles of rubratoxin B per mole of ovalbumin. The conjugate was used for immunization of rabbits, and anti-rubratox