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Product identification of polyketide synthase coded by Aspergillus nidulans wA gene

✍ Scribed by Akira Watanabe; Yuya Ono; Isao Fujii; Ushio Sankawa; María E. Mayorga; William E. Timberlake; Yutaka Ebizuka


Book ID
108387064
Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
250 KB
Volume
39
Category
Article
ISSN
0040-4039

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By reconstruction and expression of the full-length WA (NWA) polyketide synthase ofA. nidulans based on the revised wA gene sequence, its product was re-identified to be a naphthopyrone compound YWA1 instead of citreoisocoumarin and its derivatives, which were found to be products of C-terminus trun

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The Aspergillus nidulans wA gene is required for synthesis of a green pigment present in the walls of mature asexual spores (conidia); wA mutants produce colorless (white) conidia. We determined the transcriptional structure and DNA sequence of the wA gene. wA consists of 5 exons separated by short