✦ LIBER ✦

Processing of RNA by cytoplasmic extract

✍ Scribed by Mukti H. Sarma

Book ID: 104738408
Publisher: Springer
Year: 1976
Tongue: English
Weight: 436 KB
Volume: 3
Category: Article
ISSN: 0301-4851
DOI: 10.1007/bf00357208

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✦ Synopsis

Nuclei are isolated from HeLa cells by a low speed centrifugation procedure. These nuclei can carry out RNA synthesis at 30 ~ The effect of cytoplasmic extract on the transport and processing of RNA is measured. In the presence of cytoplasmic extract the precursor to 4S RNA is processed to 4S RNA and an RNA species of 7-9S is transported outside the nuclei.

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