Cytoplasmic processing events in the polyadenylate region of Physarum messenger RNA
β Scribed by W. R. Jeffery; D. S. Adams; D. Noonan
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 605 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0301-4851
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## Abstract Almost all messenger RNAs carry a polyadenylate tail that is added in a postβtranscriptional reaction. In the nuclei of animal cells, the 3'βend of the RNA is formed by endonucleolytic cleavage of the primary transcript at the site of poly (A) addition, followed by the polymerisation of
A 9S RNA fraction from mouse reticulocytes, containing the active Ξ±- and Ξ²-globin mRNAs, has been isolated by hybridization of the polyadenylate regions in the mRNAs to oligo(dT)-cellulose. The adenylate-rich sequence isolated by limited RNase digestion of the globin mRNAs migrates between 4S and 5S
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